Aside from expasions to the plot before the game's beginning (e.g. Carlos being involved in a fight between William Birkin and a T-103), the comic differs by having Jill Valentine confront a Hunter β in the Raccoon Police Station, when in Mainstream canon they are only encountered near Raccoon Park. She is also able to communicate with other people with the radio in the S.T.A.R.S. office, getting through to an obese officer located somewhere out of town. The Sword of Paracelsus becomes a weapon used by U.S.S. and is depicted to be a laser cannon instead of a railgun, and it has two variants: A portable version carried and assembled by the squad, and a larger, immovable one Jill later uses to completely obliterate Nemesis-T Type.
In the series, it is revealed that there were four Nemesis-T Types being developed. All of them could remember their previous lives (the Tyrant featured in the game is revealed to have been a professional boxer in volume 10). One of them rebels, and is killed by Umbrella after a costly battle in an office block.