The crowbar is found early during Episode 1: Penal Colony in the Detention center - experiment zone and is exclusive to Moira. It is used by pressing the melee button. Compared to Claire and Barry's knives, the crowbar is slower but has better reach. It allows Moira to perform follow-up melee attacks when enemies are downed as opposed to the knives which can't without the Follow-Up skill. In Extra Episode: The Struggle, the crowbar allows Moira to use stealth attacks by approaching enemies from behind while they are not alerted. Moira cannot perform a stealth attack on enemies in the main story, but she can stun them by attacking them from behind.
Because of its swing arc, the crowbar has a tendency to hit Afflicted in the head with regularly aimed attacks doing extra damage.
As a key item, the crowbar is required to be used (via the interact button) to pry boards off of doors and allow access to areas that Claire and Moira previously couldn't enter.
The "Batter Up" skill increases the attack power of Moira's crowbar and "Crowbar Finisher" allows BP to be gained by using a follow-up attack.