(Capcom's primary storyline)
In the post-credit cutscene, Lottie can be seen in Natalia's room, suggesting the Burtons had found a replacement for Natalia.
During Extra Episode: Little Miss, Natalia and Dark Natalia (Alex Wesker's Manifestation/Consciousness in Natalia) go on a search to find Lottie who leaves several clues to her whereabouts through postcards. Natalia describes Lottie as being "the only good in her life" and one of her only friends. She is desperate in the search for Lottie as she doesn't want to lose her like she lost her parents.
Upon finding Lottie, the bear uncharacteristically tells Natalia "Gosh-arooney, you little BITCH! I was supposed to win this game of tug-o-war!" with her voice changing near the end, then she laughs maniacally before falling limp and Natalia is in shock to witness this behaviour, with Dark Natalia then proceeding to pick her up and telling Natalia: "But there's still plenty of time to get under your skin, next time you won't see me coming", implying that Lottie here was a manifestation of Dark Natalia
- One of Natalia's DLC outfits is the "Lottie Suit", where Natalia dresses up as a life sized version of her beloved bear.
- Lottie is playable in the Raid Mode as a costume for Anonymous. An alternate white version of Lottie known as "White Lottie" is also featured as a costume. These versions are life sized compared to the stuffed bear Natalia owns. They use voice overs from the Little Miss episode. This leads to some odd limitations such as Lottie giggling when she dies.
- In the Resident Evil 2 remake, during the Sherry segment within the Raccoon City Orphanage, a drawing of what looks to be Lottie can be seen on one of the drawing boards.
- One of the PlayStation Network avatars for Resident Evil: Revelations 2 is of Lottie. However, the online store erroneously names it "Lotti".