Dr. William Birkin, the lead researcher behind the Golgotha Virus, fell under the suspicions of Umbrella HQ. An attempt to remove him from the project failed when he ordered his staff to ignore messages from HQ regarding the project, continued development despite funding being ceased, and over a two month period had seven researchers detained or executed for spying by sending reports in to HQ. Umbrella spies within the US military soon learned that he was in contact with them and offering to sell it. After Birkin ignored a direct order to respond to an investigation committee within 24 hours, Umbrella HQ prepared to seize Golgotha by force.
On the night of 22 September, 1st Squad's Alpha Team, under the command of "HUNK", infiltrated the lab through ceiling accessways and at 23:45 four soldiers confronted Dr. Birkin as he placed the Golgotha samples in a storage case. Under arrest, he resisted and drew a handgun. He was promptly shot repeatedly from an MP5 by J. Martinez, against Umbrella's orders. The storage case, unscathed by the attack, was seized by A. Kirkpatrick. Dr. Birkin was able to inject himself with a single remaining sample of Golgotha which the U.S.S. failed to recover. Several U.S.S. soldiers occupying NEST were killed in his subsequent rampage through the complex, but finally caught up to the four U.S.S. soldiers of Alpha Team and the Bravo Team compatriots in the sewers. With Kirkpatrick injured, the storage case was opened for him to consume more samples of Golgotha. Samples of the newly-modified Epsilon strain of t-Virus were also released from the case, and the samples were consumed by rats which subsequently spread their infection to Raccoon City's drinking water.
For several days, HUNK, who had escaped without serious injury with a Golgotha sample, made his way around the increasingly chaotic city, but was only able to get back in contact with the evacuation helicopter on the night of 29/30 September.