The Samurai Edge makes an appearance in Resident Evil 5. It is seen used by Albert Wesker during his fight with Chris. He draws it out on Chris during the fight on the aircraft. He points it to Chris' head and says a few lines, before getting interrupted by Sheva stabbing him in the forearm with her knife. After more fighting, the Samurai Edge is shot out of Weskers hand by Sheva. It is presumably lost shortly after the aircraft crashes into a Volcano.
The Samurai Edge is only usable in Mercenaries and Mercenaries Reunion Mode. It is used by Wesker's S.T.A.R.S. Outfit and Barry Burton character in reunion.